|
Status |
Public on Jan 08, 2010 |
Title |
T1792A1 |
Sample type |
RNA |
|
|
Source name |
primary colorectal adenocarcinoma
|
Organism |
Homo sapiens |
Characteristics |
Location: Right; DukesStage: D; Age_Diag: 88; Gender: M; DFS_Time: NA; DFS_Cens: NA; AdjXRT: N; AdjCTX: N
|
Biomaterial provider |
H Lee Moffitt Cancer Center
|
Extracted molecule |
total RNA |
Extraction protocol |
Surgically isolated colorectal cancers were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using Trizol reagent (Invitrogen).
|
Label |
biotin
|
Label protocol |
Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Location: Right; DukesStage: D; Age_Diag: 88; Gender: M; DFS_Time: NA; DFS_Cens: NA; AdjXRT: N; AdjCTX: N
|
Data processing |
Use the simpleaffy package in R/Bioconductor to calculate MAS5.0 calls. These values were subsequently normalized using quantile normalization.
|
|
|
Submission date |
Jan 08, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
Oliver Sieber |
Phone |
61-3-9341-3155
|
Fax |
61-3-9341-3104
|
URL |
http://www.ludwig.edu.au
|
Organization name |
Ludwig Institute for Cancer Research
|
Department |
Melbourne Tumour Biology Branch
|
Lab |
LCCI laboratory
|
Street address |
6th floor, Centre for Medical Research, Royal Melbourne Hospital, Royal Parade
|
City |
Parkville |
State/province |
Victoria |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE14333 |
Expression data from 290 primary colorectal cancers |
|
Relations |
Reanalyzed by |
GSE119087 |