|
| Status |
Public on Feb 20, 2019 |
| Title |
Italia_ OI_ rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Italia, OI, rep3
|
| Organism |
Vitis vinifera |
| Characteristics |
tissue: leaf apex
irrigation treatment: Over Irrigation (OI)
cultivar: Italia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 0.1 g of shoot apex collected at 10% veraison with Total RNA Isolation Mini Kit (Agilent Technologies). RNA integrity was assessed by automated gel electrophoresis on 2100 Bioanalyzer (Agilent Technologies, Amstelveen, Netherlands).
|
| Label |
Cy3
|
| Label protocol |
Agilent Technologies_One-Color Microarray-based_Gene expression Analyses_protocol version 6.9.1,
|
| |
|
| Hybridization protocol |
Agilent Technologies_One-Color Microarray-based_Gene expression Analyses_protocol version 6.9.1,
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) _using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
Gene expression
|
| Data processing |
Array images were analyzed using Agilent Feature Extraction software version 10.7.1.1 (Agilent Technologies, Santa Clara, CA) using default parameters (Protocol name_GE1_107_Sep09_grid name_028416_D_F_20100513) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Feb 04, 2019 |
| Last update date |
Feb 21, 2019 |
| Contact name |
Maria Francesca Cardone |
| E-mail(s) |
mariafrancesca.cardone@crea.gov.it
|
| Organization name |
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA)
|
| Department |
Centro di ricerca Viticoltura ed Enologia (CREA-VE)
|
| Street address |
via Casamassima 148
|
| City |
Turi |
| State/province |
Bari |
| ZIP/Postal code |
70010 |
| Country |
Italy |
| |
|
| Platform ID |
GPL26142 |
| Series (1) |
| GSE126052 |
Effect of different irrigation supply on gene expression of two table grape cultivars |
|
