|
| Status |
Public on Jun 30, 2010 |
| Title |
OS18 Replicate 1; conventional osteosarcoma tissue |
| Sample type |
RNA |
| |
|
| Source name |
human osteosarcoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
conventional osteosarcoma tissue, male, 7 years, femur, grade 2
|
| Biomaterial provider |
Charite Universitätsmedizin Berlin
|
| Treatment protocol |
The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
|
| Growth protocol |
Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
According to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
According to the standard Affymetrix protocol
|
| Scan protocol |
According to the standard Affymetrix protocol
|
| Description |
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Data processing |
According to Gcos1.4 protocol
|
| |
|
| Submission date |
Jan 09, 2009 |
| Last update date |
Jan 04, 2010 |
| Contact name |
Raphaela Fritsche |
| E-mail(s) |
raphaela.fritsche@charite.de
|
| Organization name |
Charitè Universitätsklinikum Charite
|
| Department |
Institute of Pathology
|
| Lab |
Systems Biology of Molecular Networks
|
| Street address |
Chariteplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE14359 |
Expression data from conventional osteosarcoma compared to primary non-neoplastic osteoblast cells |
|
