|
| Status |
Public on Jan 10, 2009 |
| Title |
Liver_infected_4weeks_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver homogenate, infected, 4 weeks
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: Female
Age: 6 weeks
Tissue: Liver
Treatment: 4 weeks post infection 20 cercariae
|
| Treatment protocol |
Mice were euthanized and their livers were perfused with chilled PBS. Liver tissue was collected and stored in RNAlater for microarray analysis or frozen for egg extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100mg of liver tissue or purified schistosome eggs by phase separation with Trizol (Invitrogen, USA) and column chromatography using an Rneasy Mini kit (Qiagen Inc, USA), RNA quantity was measured using a Nanodrop (Nanodrop Technologies, USA) and quality was assessed using an Agilent Bioanalyzer Nanochip (Agilent Technologies, USA)
|
| Label |
Cy3
|
| Label protocol |
cRNA was synthesised from 250ng of pooled total RNA with an Illumina Total Prep RNA Amplification Kit (Ambion Inc., USA) according to the manufacturers instructions
|
| |
|
| Hybridization protocol |
cRNA was hybridised to Illumina mouse-6 v1.1 expression beadchips according to the manufacturers instructions
|
| Scan protocol |
Microarrays were scanned using a Beadstation 500 at Green factor level 2
|
| Description |
Gene expression in the liver of C57BL/6 mice 4 weeks post infection with Schistosoma japonicum
|
| Data processing |
Data was analysed using BeadStudio and Genespring GX
|
| |
|
| Submission date |
Jan 09, 2009 |
| Last update date |
Jan 09, 2009 |
| Contact name |
Melissa Louise Burke |
| E-mail(s) |
melissa.burke@qimr.edu.au
|
| Organization name |
Queensland Institute of Medical Research
|
| Department |
Division of Infectious Diseases and Immunology
|
| Lab |
Molecular Parasitology
|
| Street address |
300 Herston Road
|
| City |
Herston |
| State/province |
QLD |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE14367 |
Temporal Expression of Chemokines Dictates the Hepatic Inflammatory Infiltrate in a Murine Model of Schistosomiasis. |
|
