|
| Status |
Public on Feb 12, 2019 |
| Title |
APA14_S8_L001_R1_001 |
| Sample type |
SRA |
| |
|
| Source name |
aldosterone-producing adenoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma
method: EDTA-anticoagulated plasma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
EDTA-anticoagulated plasma samples were isolated by QIAGEN miRNeasy Serum/Plasma Kit
Library was made by QIAseq microRNA Library Kit (by Qiagen)
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
trimming of adapters using cutadapt (http://cutadapt.readthedocs.io/en/stable/guide.html).
Reads with less than 16 bp insert sequences or with less than 10 bp Unique Molecular Index (UMI) were discarded.
Alignment of reads was performed using bowtie (http://bowtie-bio.sourceforge.net/index.shtml),
miRbase V21 was used for miRNAs.
Genome_build: miRbase V21
Supplementary_files_format_and_content: .xls file was generated by Qiagen online primary data analysing tool: (http://ngsdataanalysis.sabiosciences.com/QIAseqmiRNA).
|
| |
|
| Submission date |
Feb 11, 2019 |
| Last update date |
Feb 12, 2019 |
| Contact name |
Abel Decmann |
| E-mail(s) |
decabel@gmail.com
|
| Organization name |
Semmelweis University
|
| Department |
Department of Internal Medicine and Oncology
|
| Lab |
Endocrine Genetics
|
| Street address |
Korányi str. 2a
|
| City |
Budapest |
| ZIP/Postal code |
1083 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL15520 |
| Series (1) |
| GSE126386 |
Circulating miRNA expression profiling in primary aldosteronism |
|
| Relations |
| BioSample |
SAMN10910546 |
| SRA |
SRX5359095 |
