|
| Status |
Public on Feb 16, 2019 |
| Title |
Right carotid-tPA-4days-Replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Wistar Kyoto rats common carotid arteries
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain/background: Wistar-Kyoto
gender: Male
age: 3 months
tissue: Right carotid
surgery: Balloon catheter injury of left carotid
treatment: tPA applied to left carotid
time point: 4 days
|
| Treatment protocol |
Ketamine anesthesia was used (100 mg/kg body wt) and the balloon catheter (Fogarty 2F) injury of rats left common carotid arteries was performed. Following balloon catheter injury, 20 nmol/kg of recombinant tissue-type plasminogen activator dissolved in pluronic gel or 500 μl of plain Pluronic gel, F-127 (BASF) was applied to the adventitial side of the left common carotid arteries.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For microarrays, 1 and 4 days after the operation carotids were rapidly removed and frozen in liquid nitrogen. Total RNA was extracted from carotid arteries of injured, sham-operated and intact rats.
Total RNA was isolated with the use of Qiagen RNeasy Micro kit, the quality of RNA was confirmed. For each probe vessels from 3 rats were pooled.
|
| Label |
Biotin
|
| Label protocol |
Labeled with biotin.
|
| |
|
| Hybridization protocol |
Microarray analysis was performed at the University of Rochester Microarray Core Facility in accordance to Affymetrix recommendations using Affymetrix rat genome U34A oligonucleotide microarrays with 8799 known genes and expressed sequence tags (ESTs) (Santa Clara, CA). Streptavidin phycoerythrin stain (SAPE, Molecular Probes) was used.
|
| Scan protocol |
Target hybridization was detected and quantified with a Gene Array Scanner (Hewlett Packard/Affymetrix). “Array performance” was assessed. Hybridization efficiency and sensitivity were controlled using control transcripts spiked into the hybridization cocktail.
|
| Description |
3-month-old male Wistar-Kyoto rats.
|
| Data processing |
Inter-array variability was assessed by Microarray Analysis Suite 5.0. For data normalization global scaling with target intensity of 500 across all probe sets was used.
|
| |
|
| Submission date |
Feb 15, 2019 |
| Last update date |
Feb 16, 2019 |
| Contact name |
Olga Plekhanova |
| Organization name |
Lomonosov Moscow State University
|
| Street address |
Lomonosovsky prospect, 27-1
|
| City |
Moscow |
| ZIP/Postal code |
119991 |
| Country |
Russia |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE126627 |
Oligonucleotide Microarrays Identified Potential Regulatory Genes Related To Early Outward Arterial Remodeling Induced By Tissue Plasminogen Activator |
|
