|
| Status |
Public on Feb 21, 2022 |
| Title |
OsUBA-RNA interferenced rice,rep1 |
| Sample type |
RNA |
| |
|
| Source name |
OsUBA-RNA interferenced rice seedling
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: four-leaf seedlings
|
| Treatment protocol |
no treatment.
|
| Growth protocol |
Rice seedlings of OsUBA-overexprssed, OsUBA-RNA interferenced,and the wild type were normally-grown at 28 ℃ until the fourth leave arises
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 21, 2019 |
| Last update date |
Feb 21, 2022 |
| Contact name |
Caili Bi |
| E-mail(s) |
beicaili@sina.com
|
| Organization name |
Hebei Normal University
|
| Department |
College of life sciences
|
| Street address |
No.20 Road East. 2nd Ring South, Yuhua District
|
| City |
Shijiazhuang |
| State/province |
Hebei |
| ZIP/Postal code |
050024 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE126878 |
Expression data from OsUBA-overexpressed, RNA interferenced, and wild-type rice seedlings |
|
