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Sample GSM361998 Query DataSets for GSM361998
Status Public on Jan 21, 2009
Title naïve-H3K27me3
Sample type SRA
Source name Naive cells
Organism Mus musculus
Characteristics naïve-H3K27me3
Treatment protocol Treatment protocol as described in Wei et al., 2009 (PMID:19144320)
Growth protocol Growth protocol as described in Wei et al., 2009 (PMID:19144320)
Extracted molecule genomic DNA
Extraction protocol Extract protocol as described in Wei et al., 2009 (PMID:19144320)
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Description Chromatin IP
T cells were treated with MNase to generate approximately 80% mononucleosomes and 20% dinucleosomes. Chromatin from 2 × 107 cells was used for each ChIP experiment, which yielded approximately 100 ng of DNA. Antibodies against histone H3K4me3 (ab8580, Abcam) and H3K27me3 (07-449, Upstate) were used. The ChIP DNA fragments were blunt-ended, ligated to the Solexa adaptors, and sequenced with the Illumina 1G Genome Analyzer.
Data processing Sequence reads were obtained and mapped to the mouse (February, 2006) genomes using the Illumina Genome Analyzer Pipeline. More details as described in Wei et al., 2009 (PMID:19144320)
Submission date Jan 21, 2009
Last update date May 15, 2019
Contact name Weiqun Peng
Organization name The Georege Washington University
Street address 725 21st street, NW
City DC
ZIP/Postal code 20052
Country USA
Platform ID GPL9185
Series (1)
GSE14254 Global Mapping of Histone H3 K4 and K27 Trimethylation: Lineage Fate Determination of Differentiating CD4+ T Cells
SRA SRX003808
BioSample SAMN02195434
Named Annotation GSM361998.bed.gz

Supplementary file Size Download File type/resource
GSM361998.bed.gz 106.6 Mb (ftp)(http) BED
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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