|
| Status |
Public on Aug 31, 2019 |
| Title |
N1ICD, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
N1ICD-1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 184-hTerts
|
| Treatment protocol |
Cells were lentivirally transduced and after 48 hours, the transduded cells expressing green fluorescent protein (GFP) are sorted via FACS for RNA isolation
|
| Growth protocol |
184-hTert cells were grown in DMEM:F12 1:1 media supplemented with 10ng EGF, 25 μg Insulin, 500μg hydrocortisone, 2.5ng sodium selenate, 400μg G418, 0.15U prolactin, 1.6 μg transferrin and 10nM isoproterenol and were subcultured every 2-3 days (before reaching confluency). Both the cell types were maintained in a humidified chamber at 37c and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was done using Trizol, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
|
| |
|
| Hybridization protocol |
5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Human Gene 2.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Partek Genomics Suite using the RMA normalization method
|
| |
|
| Submission date |
Feb 26, 2019 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Afshin Raouf |
| E-mail(s) |
afshin.raouf@umanitoba.ca
|
| Phone |
2049757704
|
| Organization name |
University of Manitoba
|
| Department |
Immunology
|
| Street address |
471 Apotex centre, 750 McDermot Avenue
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3E0T5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE127236 |
Identification of NOTCH3 unique target genes |
|
