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Sample GSM3634286 Query DataSets for GSM3634286
Status Public on Jan 31, 2022
Title dairy goat mammary gland_H1
Sample type SRA
 
Source name dairy goat mammary gland
Organism Capra hircus
Characteristics tissue: Mammary gland
developmental stage: lactation
Sex: female
treatment: high dose of glucose
Extracted molecule total RNA
Extraction protocol Mammary gland were biopsied, moved into liquid nitrogen, and RNA was extracted using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads were calculated.
Genome_build: mm8
Supplementary_files_format_and_content: tab-delimited text files include Reads values for each Sample.
 
Submission date Feb 28, 2019
Last update date Jan 31, 2022
Contact name Jie Cai
E-mail(s) 876418837@qq.com
Phone 18268808627
Organization name Zhejiang University
Street address Yuhangtang Rd
City Hangzhou
ZIP/Postal code 310058
Country China
 
Platform ID GPL19149
Series (1)
GSE127429 Glucose infusion into dairy goat mammary gland
Relations
BioSample SAMN11032839
SRA SRX5443937

Supplementary file Size Download File type/resource
GSM3634286_H1.txt.gz 1.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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