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Status |
Public on Oct 29, 2019 |
Title |
Patient 56, tubulointerstitium |
Sample type |
RNA |
|
|
Source name |
Patient 56, tubulointersticial tissue
|
Organism |
Homo sapiens |
Characteristics |
subject id: Patient 56 class: 4 subclass: 1 tissue: tubulointersticial tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from micro-dissected glomerular and tubulointerstitial comparments by silica gel columns and diluted in 30 μL RNase-free water.
|
Label |
biotin
|
Label protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
|
|
Hybridization protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
Scan protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
Data processing |
Raw array data (CEL files) were loaded into the R statistical environment (v3.3.1) using the affy package (v1.48.0) of the BioConductor library. Data was investigated for distributional homogeneity, then pre-processed using the RMA algorithm. An updated Probe to Entrez Gene ID map, hta20hsentrezgcdf (v20.0.0), was used for annotation.
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Submission date |
Mar 04, 2019 |
Last update date |
Oct 29, 2019 |
Contact name |
Stephenie Prokopec |
E-mail(s) |
stephenie.prokopec@oicr.on.ca
|
Organization name |
Ontario Institute for Cancer Research
|
Street address |
661 University Avenue, Suite 510
|
City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 0A3 |
Country |
Canada |
|
|
Platform ID |
GPL24299 |
Series (1) |
GSE127797 |
The Molecular Phenotype of Lupus Nephritis Histological Lesions |
|