NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM364037 Query DataSets for GSM364037
Status Public on Jun 27, 2011
Title small airways, COPD 130
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 57
Sex: F
Ethnic group: white
Smoking status: COPD, GOLD-II, 38.5 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description COPD-130_sm
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 23, 2009
Last update date Jun 27, 2011
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (2)
GSE20257 Smoking-induced Disarray of the Apical Junctional Complex Gene Expression Architecture in the Human Airway Epithelium
GSE21359 Association of CXCL14 in the Human Airway Epithelium with Chronic Obstructive Lung Disease and Lung Cancer

Data table header descriptions
ID_REF
VALUE MAS5-calculated signal intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1693.11 P 0.000195116
AFFX-BioB-M_at 2900.29 P 4.42873e-05
AFFX-BioB-3_at 1699.49 P 4.42873e-05
AFFX-BioC-5_at 4682.71 P 5.16732e-05
AFFX-BioC-3_at 6166.26 P 4.42873e-05
AFFX-BioDn-5_at 10495.4 P 4.42873e-05
AFFX-BioDn-3_at 20577.7 P 4.42873e-05
AFFX-CreX-5_at 60701.4 P 5.16732e-05
AFFX-CreX-3_at 62027.8 P 4.42873e-05
AFFX-DapX-5_at 53.5786 A 0.262827
AFFX-DapX-M_at 14.8607 A 0.783476
AFFX-DapX-3_at 4.40228 A 0.988616
AFFX-LysX-5_at 32.0757 A 0.440646
AFFX-LysX-M_at 9.58049 A 0.921998
AFFX-LysX-3_at 7.44943 A 0.772364
AFFX-PheX-5_at 11.4535 A 0.699394
AFFX-PheX-M_at 4.21136 A 0.984817
AFFX-PheX-3_at 40.4607 A 0.470241
AFFX-ThrX-5_at 5.63564 A 0.937071
AFFX-ThrX-M_at 9.91582 A 0.804734

Total number of rows: 54675

Table truncated, full table size 1628 Kbytes.




Supplementary file Size Download File type/resource
GSM364037.CEL.gz 8.1 Mb (ftp)(http) CEL
GSM364037.CHP.gz 13.3 Mb (ftp)(http) CHP
Processed data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap