|
| Status |
Public on May 10, 2011 |
| Title |
control |
| Sample type |
RNA |
| |
|
| Source name |
Rattus norvegicus - subventricular zone - unstimulated
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: 3 months
tissue: subventricular zone
|
| Treatment protocol |
During this time period, cells were treated with either HCl/BSA (control) or 10 ng/ml TGFbeta1 (TGFbeta1) 3 times per week.
|
| Growth protocol |
Adult rat subventricular zone cells were grown under proliferating conditions in the presence of EGF and FGF for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with Rneasy Midi Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on GeneChip Rat Expression Set 230 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from adult rat subventricular zone cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 23, 2009 |
| Last update date |
May 10, 2011 |
| Contact name |
Dietrich Trümbach |
| E-mail(s) |
dietrich.truembach@helmholtz-muenchen.de
|
| Organization name |
German Research Center for Environmental Health
|
| Department |
Institute for Developmental Genetics
|
| Street address |
Ingolstädter Landstraße 1
|
| City |
München-Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE14556 |
Expression data from adult rat subventricular zone cells |
|
