|
| Status |
Public on Dec 30, 2013 |
| Title |
VEGF-induced CAM 2 |
| Sample type |
RNA |
| |
|
| Source name |
CAM was isolated from a chick embryo at developmental day 14
|
| Organism |
Gallus gallus |
| Characteristics |
'tissue: chorio-allantoic membrane' 'Embryo age: E14'
|
| Biomaterial provider |
Martin Hagedorn
|
| Treatment protocol |
Thermanox discs were deposited on the surface of the intact CAM on E13. CAM areas under the disc were excised on E14 and snap-frozen in liquid nitrogen prior to mRNA isolation
|
| Growth protocol |
Chick embryos were incubated at 37.8ºC and 75% humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CAMs were immediately snap-frozen in liquid nitrogen after excision. Total
RNA was isolated using RNeasy mini kits (Qiagen) according to the
manufacturer's instructions and quantified using a NanoDrop
spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) 'small sample protocol'
|
| |
|
| Hybridization protocol |
Hybridization and washing was carried out on a GeneChip (Affymetrix) station, washing according to the manufacturer’s protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
A pool of three normal chick-chorio-allantoic membrane fragements were compared to three individual CAMs treated with 3 micrograms of human recombinant VEGF for 24h (VEGF dried on a Thermanox disc).
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 27, 2009 |
| Last update date |
Dec 30, 2013 |
| Contact name |
Martin Hagedorn |
| E-mail(s) |
m.hagedorn@angio.u-bordeaux1.fr
|
| Organization name |
University Bordeaux 1
|
| Department |
Biology
|
| Lab |
INSERM U920
|
| Street address |
Av des Facultes
|
| City |
Talence |
| ZIP/Postal code |
33405 |
| Country |
France |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE14587 |
Genes induced by VEGF on the chick CAM after 24h |
|
