|
| Status |
Public on Jan 29, 2009 |
| Title |
NR4 (COG study 8704) |
| Sample type |
RNA |
| |
|
| Source name |
Primary T-ALL cells; newly diagnosed patient; no response (induction failure)
|
| Organism |
Homo sapiens |
| Characteristics |
Newly diagnosed patient
|
| Treatment protocol |
COG study 8704
|
| Growth protocol |
Derived from bone marrow in newly diagnosed patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasyMini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotin-labed cRNA from 2.5 micrgrams of tissue using a two-step method usng Affymetrix technical approach
|
| |
|
| Hybridization protocol |
15 ug aliquots of fragmented, labelled cRNA were hybridized to the U133A chip (Affymetrix)
|
| Scan protocol |
Affymerix microarray scanner (Hewlett Packard), gene fluorescence was captured using GeneChip software.
|
| Description |
NR =no response (induction failure)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and the RMA normalization method.
|
| |
|
| Submission date |
Jan 28, 2009 |
| Last update date |
Jan 28, 2009 |
| Contact name |
Stuart S. Winter |
| E-mail(s) |
swinter@salud.unm.edu
|
| Phone |
505 272-4461
|
| Organization name |
University of New Mexico Health Sciences Center
|
| Department |
Pediatrics
|
| Street address |
MSC 10 5590
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131-5311 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE14613 |
Microarray analyses of induction failure in T-ALL (COG study 8704) |
| GSE14618 |
Microarray analyses of induction failure in T-ALL |
|
