|
Status |
Public on Jan 29, 2009 |
Title |
C2 (COG study 8704) |
Sample type |
RNA |
|
|
Source name |
Primary T-ALL cells; newly diagnosed patient; Complete Continous Remission
|
Organism |
Homo sapiens |
Characteristics |
Newly diagnosed patient
|
Treatment protocol |
COG study 8704
|
Growth protocol |
Derived from bone marrow in newly diagnosed patients
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasyMini Kit (Qiagen)
|
Label |
Biotin
|
Label protocol |
Biotin-labed cRNA from 2.5 micrgrams of tissue using a two-step method usng Affymetrix technical approach
|
|
|
Hybridization protocol |
15 ug aliquots of fragmented, labelled cRNA were hybridized to the U133A chip (Affymetrix)
|
Scan protocol |
Affymerix microarray scanner (Hewlett Packard), gene fluorescence was captured using GeneChip software.
|
Description |
C= Complete Continous Remission
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and the RMA normalization method.
|
|
|
Submission date |
Jan 28, 2009 |
Last update date |
Jan 28, 2009 |
Contact name |
Stuart S. Winter |
E-mail(s) |
swinter@salud.unm.edu
|
Phone |
505 272-4461
|
Organization name |
University of New Mexico Health Sciences Center
|
Department |
Pediatrics
|
Street address |
MSC 10 5590
|
City |
Albuquerque |
State/province |
NM |
ZIP/Postal code |
87131-5311 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (2) |
GSE14613 |
Microarray analyses of induction failure in T-ALL (COG study 8704) |
GSE14618 |
Microarray analyses of induction failure in T-ALL |
|