|
| Status |
Public on Nov 13, 2009 |
| Title |
Male corneal epithelial cells, biological rep 3 (Affymetrix) |
| Sample type |
RNA |
| |
|
| Source name |
Corneoscleral rim scrappings from a male donor age 50.
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell type: corneal epithelial cells
cell age: 50
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was twice purified with Trizol following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on an Affymetrix GeneChip Human Genome U133A 2.0 Array in an Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
|
| Scan protocol |
GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
|
| Description |
Corneoscleral rim scrappings from a male donor age 50.
|
| Data processing |
Data were analyzed with GCOS software using the default settings. The default scaling produced a trimmed mean intensity of 500.
|
| |
|
| Submission date |
Jan 28, 2009 |
| Last update date |
Nov 13, 2009 |
| Contact name |
David A Sullivan |
| E-mail(s) |
david.sullivan@schepens.harvard.edu
|
| Phone |
(617) 912-0287
|
| Fax |
(617) 912-0101
|
| Organization name |
Schepens Eye Research Institute
|
| Lab |
David Sullivan
|
| Street address |
20 Staniford Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE14621 |
Influence of Sex on Gene Expression in Human Corneal Epithelial Cells |
|
