|
| Status |
Public on Oct 07, 2019 |
| Title |
Rhode Island Red-RIR177 |
| Sample type |
genomic |
| |
|
| Source name |
Rhode Island Red
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Blood
|
| Treatment protocol |
None
|
| Growth protocol |
chickens were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from peripheral blood using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
The array image was acquired using Affymetrix Operating Software (AxAS version 4.0.1).
Genotype Call (SNP call): A/T/C/G
|
| |
|
| Submission date |
Mar 06, 2019 |
| Last update date |
Oct 07, 2019 |
| Contact name |
Changsheng Nie |
| E-mail(s) |
changshengnie@gmail.com
|
| Organization name |
China Agricultural University
|
| Department |
Animal Genetics and Breeding
|
| Street address |
Yuanmingyuan road 2#
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL23815 |
| Series (1) |
| GSE127968 |
Affymetrix SNP array data for chicken samples |
|
