|
| Status |
Public on Mar 08, 2019 |
| Title |
flcn4 |
| Sample type |
RNA |
| |
|
| Source name |
flcn-1 L4-young adult animals
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: RB1035
genotype/variation: flcn
developmental stage: L4-young adult
tissue: whole body
|
| Treatment protocol |
Synchronized animals grown on NGM OP50 plates were harvested at the L4 - young adult stage.
|
| Growth protocol |
Synchronized Wt and flcn-1 animals were plated on OP50 plates and grown for 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and purified using Qiagen columns.
|
| Label |
Cy3
|
| Label protocol |
Cyanine 3-labeled CTP cRNA was produced from total RNA using the Low Input Quick Amp Labeling Kit, according to manufacturer’s instructions (Agilent Technologies, Inc).
|
| |
|
| Hybridization protocol |
The labeled cRNA was normalized at 1.65 µg, fragmented and hybridized to C. Elegans 4x44k oligonucleotide microarrays as per manufacturer's instructions.
|
| Scan protocol |
We used an Agilent Microarray Scanner System, an Agilent Scanner C type.
|
| Description |
Biological replicate 4 of 4. Flcn-mutant.
|
| Data processing |
Flexarray Software was used for background subtraction and normalization using the vsn algorithm.
|
| |
|
| Submission date |
Mar 07, 2019 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Alain Pacis |
| E-mail(s) |
alain.pacis@mcgill.ca
|
| Organization name |
Canadian Centre for Computational Genomics (C3G)
|
| Street address |
740 Dr Penfield Ave
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3A 0G1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL11346 |
| Series (2) |
| GSE126656 |
The transcription factors TFEB and TFE3 link the FLCN-AMPK signaling axis to innate immune response and pathogen resistance |
| GSE128029 |
The transcription factors TFEB and TFE3 link the FLCN-AMPK signaling axis to innate immune response and pathogen resistance [array] |
|
