|
| Status |
Public on Sep 15, 2020 |
| Title |
LSEC_early_2 |
| Sample type |
RNA |
| |
|
| Source name |
liver endothelium derived from preneoplastic liver
|
| Organism |
Mus musculus |
| Characteristics |
cell type: total LSEC
genotype: TG
timepoint: Early
|
| Growth protocol |
Primary liver endothelial cells were isolated by a collagenase digest, gradient centrifugation and magnetic labeling. Magnetically enriched Ecs were used for FACS and dead live staining. RNA was extracted directly after the cell sorting procedure.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure extraction kit (Life technologies) according to the manufacturer*s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
CD31+ CD146+ Lyve1+ Retinoid-
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 20 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Mar 08, 2019 |
| Last update date |
Sep 16, 2020 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22598 |
| Series (2) |
| GSE128044 |
YAP orchestrates heterotypic endothelial cell communication via HGF/c-MET signaling in liver tumorigenesis |
| GSE128047 |
An endothelial subtype-specific communication network orchestrates capillarization in liver tumorigenesis via the Hgf/c-Met pathway |
|
