|
Status |
Public on Aug 30, 2019 |
Title |
Muscle_IBM-061 |
Sample type |
RNA |
|
|
Source name |
Inclusion body myositis_muscle
|
Organism |
Homo sapiens |
Characteristics |
disease state: Inclusion body myositis tissue: Muscle
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RNAeasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133A Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
Data processing |
The data were analyzed using GCRMA normalization.
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|
|
Submission date |
Mar 18, 2019 |
Last update date |
Sep 01, 2019 |
Contact name |
Steven A Greenberg |
E-mail(s) |
sagreenberg@partners.org
|
Organization name |
Brigham and Women's Hospital
|
Department |
Neurology
|
Street address |
60 Fenwood Road
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
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