|
| Status |
Public on Nov 13, 2009 |
| Title |
Cultured male corneal epithelial cells, biological rep 2 (Codelink 18.5K) |
| Sample type |
RNA |
| |
|
| Source name |
Cultured male corneal epithelial cells from a male donor age 60.
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: cultured corneal epithelial cells
cell age: 60
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was twice purified with Trizol following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 18 hr at 37C on GE Healthcare/Amersham Biosciences CodeLink UniSet Human 20K I Bioarray. Bioarrays were washed and stained with streptavidin-Alexa 647 and washed again.
|
| Scan protocol |
Bioarrays were scanned with a ScanArray Express HT scanner using ScanArray Express software.
|
| Description |
Cultured male corneal epithelial cells from a male donor age 60. Outgrowths were cultured from cornea rims in keratinocyte serum-free medium containing 50 µg/ml bovine pituitary extract and 5 ng/ml epidermal growth factor. At 70% confluence the corneal epithelial cells were lifted with trypsin and seeded onto collagen coated plates. This first passage was then grown to confluence in the medium above and the RNA isolated.
|
| Data processing |
Data were analyzed with the CodeLink Image Analysis software using the default settings and normalized using global scaling to a median target intensity on each array of 1.0.
|
| |
|
| Submission date |
Feb 05, 2009 |
| Last update date |
Nov 13, 2009 |
| Contact name |
David A Sullivan |
| E-mail(s) |
david.sullivan@schepens.harvard.edu
|
| Phone |
(617) 912-0287
|
| Fax |
(617) 912-0101
|
| Organization name |
Schepens Eye Research Institute
|
| Lab |
David Sullivan
|
| Street address |
20 Staniford Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL8159 |
| Series (1) |
| GSE14621 |
Influence of Sex on Gene Expression in Human Corneal Epithelial Cells |
|
