|
| Status |
Public on Nov 13, 2009 |
| Title |
Female corneal epithelial cells, biological rep 3 (Codelink 19.2K) |
| Sample type |
RNA |
| |
|
| Source name |
Corneoscleral rim scrappings from a female donor age 50.
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: corneal epithelial cells
cell age: 50
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was twice purified with Trizol following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 18 hr at 37C on GE Healthcare/Amersham Biosciences CodeLink UniSet Human 20K I Bioarray. Bioarrays were washed and stained with streptavidin-Alexa 647 and washed again.
|
| Scan protocol |
Bioarrays were scanned with a ScanArray Express HT scanner using ScanArray Express software.
|
| Description |
Corneoscleral rim scrappings from a female donor age 50.
|
| Data processing |
Data were analyzed with the CodeLink Image Analysis software using the default settings and normalized using global scaling to a median target intensity on each array of 1.0.
|
| |
|
| Submission date |
Feb 05, 2009 |
| Last update date |
Nov 13, 2009 |
| Contact name |
David A Sullivan |
| E-mail(s) |
david.sullivan@schepens.harvard.edu
|
| Phone |
(617) 912-0287
|
| Fax |
(617) 912-0101
|
| Organization name |
Schepens Eye Research Institute
|
| Lab |
David Sullivan
|
| Street address |
20 Staniford Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL8158 |
| Series (1) |
| GSE14621 |
Influence of Sex on Gene Expression in Human Corneal Epithelial Cells |
|
