|
| Status |
Public on Mar 20, 2019 |
| Title |
Tumor_4 |
| Sample type |
SRA |
| |
|
| Source name |
Astrocytes Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Astrocytes
barcode: BC08
batch_id: Batch1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Astrocytes were extracted by HepaCAM-Immunopanning
Library was constructed by Nanopore cDNA sequencing Kit, SQK-PCS109
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MinION |
| |
|
| Description |
BC08_Batch1
|
| Data processing |
Basecalling by Albacore 2.0.1, Quality Control, Identification of Barcoded Samples by PoreChop
Alignment by MiniMap2
Nanopore Pipeline (www.github.com/heilandd/)
Postprocessing by DESeq2 and AutoPipe (Cran)
Genome_build: GRch38
Supplementary_files_format_and_content: tab-delimited text files of counts per barcode
|
| |
|
| Submission date |
Mar 19, 2019 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Dieter Henrik Heiland |
| E-mail(s) |
dieter.henrik.heiland@uniklinik-freiburg.de
|
| Organization name |
Medical Center Freiburg
|
| Department |
Department of Neurosurgery
|
| Street address |
Breisacherstr. 64
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24106 |
| Series (1) |
| GSE128536 |
Tumor-associated Reactive Astrocytes Aid the Evolution of Immunosuppressive Environment in Glioblastoma |
|
| Relations |
| BioSample |
SAMN11166342 |
| SRA |
SRX5543174 |
