|
Status |
Public on Sep 17, 2019 |
Title |
RNAseq_CS_rep58 |
Sample type |
SRA |
|
|
Source name |
uterin carcinosarcoma
|
Organism |
Homo sapiens |
Characteristics |
anatomical site: uterus Sex: female age: 60-64
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy Micro kit (Qiagen). Stranded mRNA HT Sample Prep Kit (Illumina) was used with 200 ng of total RNA for the construction of sequencing libraries.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Illumina Casava1.8.2 software used for basecalling Sequenced reads were mapped to hg19 using bowtie2-2.2.3. Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using RSEM-1.3.0. Rsem.genes.results file has columns named TPM and FPKM. Each columns represent Transcripts Per Million mapped reads and Fragments Per Kilobase of transcript and Million mapped reads for each gene. Genome_build: hg19 Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample.
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|
|
Submission date |
Mar 20, 2019 |
Last update date |
Sep 20, 2019 |
Contact name |
Osamu Gotoh |
Organization name |
Japanese Foundation For Cancer Research
|
Street address |
Ariake 3-8-31
|
City |
Koto-ku |
State/province |
Tokyo |
ZIP/Postal code |
135-8550 |
Country |
Japan |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE128630 |
Transcriptome data of uterine and ovarian carcinosarcoma tissues derived from patients in the Cancer Institute Hospital of Japanese Foundation for Cancer Research |
|
Relations |
BioSample |
SAMN11179032 |