|
Status |
Public on Dec 22, 2009 |
Title |
Favorable Histology Wilms Tumor (WT00-276) |
Sample type |
RNA |
|
|
Source name |
Pretreatment tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
Pretreatment tumor tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by Trizol (Invitrogen, Carlsbad, CA) extraction and treated with DNase I (Roche, Indianapolis, IN).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
The labeled, fragmented cRNA was hybridized to the Affymetrix HG - U133A oligonucleotide array for 16 hours at 45°C according to Affymetrix protocol. Staining was done in the GeneChip Fluidics Station using pre-programmed Affymetrix protocols.
|
Scan protocol |
The arrays were scanned in an HP GeneArray Scanner.
|
Description |
none
|
Data processing |
Data were normalized using robust multi-array average (RMA) method. This normalized value of each probeset for each tumor is provided in the file “All Normalized Data.”
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|
|
Submission date |
Feb 09, 2009 |
Last update date |
Dec 21, 2009 |
Contact name |
Elizabeth J Perlman |
E-mail(s) |
eperlman@childrensmemorial.org
|
Organization name |
Children's Memorial Research Center
|
Department |
Pathology
|
Street address |
2373 North Lincoln Avenue, Room A.204
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60614 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE14767 |
Subsets of very low risk Wilms Tumors show distinctive gene expression, histologic, and clinical features |
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