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Sample GSM368798 Query DataSets for GSM368798
Status Public on Dec 22, 2009
Title Favorable Histology Wilms Tumor (WT00-276)
Sample type RNA
 
Source name Pretreatment tumor tissue
Organism Homo sapiens
Characteristics Pretreatment tumor tissue
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol (Invitrogen, Carlsbad, CA) extraction and treated with DNase I (Roche, Indianapolis, IN).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented cRNA was hybridized to the Affymetrix HG - U133A oligonucleotide array for 16 hours at 45°C according to Affymetrix protocol. Staining was done in the GeneChip Fluidics Station using pre-programmed Affymetrix protocols.
Scan protocol The arrays were scanned in an HP GeneArray Scanner.
Description none
Data processing Data were normalized using robust multi-array average (RMA) method. This normalized value of each probeset for each tumor is provided in the file “All Normalized Data.”
 
Submission date Feb 09, 2009
Last update date Dec 21, 2009
Contact name Elizabeth J Perlman
E-mail(s) eperlman@childrensmemorial.org
Organization name Children's Memorial Research Center
Department Pathology
Street address 2373 North Lincoln Avenue, Room A.204
City Chicago
State/province IL
ZIP/Postal code 60614
Country USA
 
Platform ID GPL96
Series (1)
GSE14767 Subsets of very low risk Wilms Tumors show distinctive gene expression, histologic, and clinical features

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 9.80093
1053_at 6.30355
117_at 5.64847
121_at 10.392
1255_g_at 4.34208
1294_at 7.20034
1316_at 6.13852
1320_at 4.72091
1405_i_at 4.30358
1431_at 4.12868
1438_at 8.35132
1487_at 7.02718
1494_f_at 6.58577
1598_g_at 8.73178
160020_at 8.89813
1729_at 7.28304
1773_at 5.9618
177_at 5.22131
179_at 9.11942
1861_at 6.01393

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.




Supplementary file Size Download File type/resource
GSM368798.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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