|
| Status |
Public on Dec 22, 2009 |
| Title |
Favorable Histology Wilms Tumor (WT00-342) |
| Sample type |
RNA |
| |
|
| Source name |
Pretreatment tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Pretreatment tumor tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol (Invitrogen, Carlsbad, CA) extraction and treated with DNase I (Roche, Indianapolis, IN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled, fragmented cRNA was hybridized to the Affymetrix HG - U133A oligonucleotide array for 16 hours at 45°C according to Affymetrix protocol. Staining was done in the GeneChip Fluidics Station using pre-programmed Affymetrix protocols.
|
| Scan protocol |
The arrays were scanned in an HP GeneArray Scanner.
|
| Description |
none
|
| Data processing |
Data were normalized using robust multi-array average (RMA) method. This normalized value of each probeset for each tumor is provided in the file “All Normalized Data.”
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Dec 21, 2009 |
| Contact name |
Elizabeth J Perlman |
| E-mail(s) |
eperlman@childrensmemorial.org
|
| Organization name |
Children's Memorial Research Center
|
| Department |
Pathology
|
| Street address |
2373 North Lincoln Avenue, Room A.204
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60614 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE14767 |
Subsets of very low risk Wilms Tumors show distinctive gene expression, histologic, and clinical features |
|
