|
| Status |
Public on Oct 01, 2009 |
| Title |
B-ALL_E2A/PBX1 2 (mRNA) |
| Sample type |
RNA |
| |
|
| Source name |
Bone Marrow
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 34
phenotype: pre B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (2-8 ug) was converted into double stranded cDNA using a cDNA Synthesis System kit (Roche Applied Science_Mannheim_Germany) and the Poly-A control transcripts (Affymetrix_Santa Clara_CA). cDNA was then used for in vitro transcription reaction using the Microarray RNA target synthesis kit (Roche Applied Science) and labeling nucleotide mix (Affymetrix) to synthesize biotinylated complementary RNA (cRNA)
|
| |
|
| Hybridization protocol |
Hybridization was performed overnight on a µParaflo microfluidic chip using a micro-circulation pump (Atactic Technologies)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000Dx scanner
|
| Description |
Gene expression data from patient with newly diagnosed ALL (percentage of leukemic cells at least 80%)
|
| Data processing |
Data were analyzed with R using functions ReadAffy and mas5 from package affy with default analysis settings.
|
| |
|
| Submission date |
Feb 17, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Teresa Colombo |
| Organization name |
Consiglio Nazionale delle Ricerche (CNR)
|
| Department |
Institute of Molecular Biology and Pathology (IBPM)
|
| Street address |
p.le A. Moro 7
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14834 |
Characterization of B- and T-lineage ALL by Integrated Analysis of microRNA and mRNA Expression Profiles |
|
| Relations |
| Reanalyzed by |
GSE119087 |
