|
| Status |
Public on Aug 29, 2019 |
| Title |
LA1-55n_shGLDC99b |
| Sample type |
RNA |
| |
|
| Source name |
LA1-55n_teton-shGLDC99_doxy-0.5µg-7d
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Neuroblastoma
cell line: LA1-55n
|
| Treatment protocol |
LA1-55n_teton-shGFP and -shGLDC99 cells were cultured n the presence of 0.5µg/ml doxycycline for 7 days
|
| Growth protocol |
LA1-55n_teton-shGFP and -shGLDC99 cells were cultured in RPMI 1640 (HyClone SH30027, Thermo Fisher Scientific) supplemented with 10% FBS (Atlanta Biologicals S11050).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
RNA expression value
|
| Data processing |
Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc., St. Louis, MO).
|
| |
|
| Submission date |
Apr 15, 2019 |
| Last update date |
Aug 29, 2019 |
| Contact name |
Han-Fei Ding |
| E-mail(s) |
hding@uabmc.edu
|
| Phone |
7067269419
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Pathology
|
| Lab |
Ding
|
| Street address |
WTI 602D, 1824 6th Avenue South
|
| City |
Birmingham |
| State/province |
Alabama |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE129807 |
Gene expression profiling of human neuroblastoma cell line LA1-55n expressing shGFP or shRNA to glycine decarboxylase (GLDC) |
|
