|
Status |
Public on Apr 15, 2009 |
Title |
H441 |
Sample type |
RNA |
|
|
Source name |
human NSCLC cell line H441
|
Organism |
Homo sapiens |
Characteristics |
cell line: H441
|
Growth protocol |
exponentially growing cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
gene expression from the human NSCLC cell line H441
|
Data processing |
Data were processed with dCHIP 1.3.1
|
|
|
Submission date |
Feb 20, 2009 |
Last update date |
Sep 01, 2016 |
Contact name |
Thomas Zander |
Organization name |
University Hospital Cologne
|
Street address |
Kerpenerstr. 62
|
City |
Cologne |
ZIP/Postal code |
50924 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE14925 |
Expression data from Non small cell lung cancer cell lines |
|
Relations |
Reanalyzed by |
GSE86363 |