|
| Status |
Public on May 20, 2009 |
| Title |
P_CD8_10 |
| Sample type |
RNA |
| |
|
| Source name |
CD8 T cells, Positive Selection
|
| Organism |
Homo sapiens |
| Characteristics |
age: 31
gender: Male
t_cell_purity: 89%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix Expression GeneChip Protocol one-cycle procedure. Between 2 and 5micrograms of RNA was used as starting material.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridised for 16 hours at 45C on Affymetrix U133Plus2 GeneChips. Chips were then washed and stained with SAPE in an Affymetrix GeneChip 450 Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Ayymetrix GeneChip 3000 scanner
|
| Description |
PS10 CD8
|
| Data processing |
Data was normalised using RMA
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Rifca Le Dieu |
| E-mail(s) |
rledieu@gmail.com
|
| Organization name |
Queen Mary University of London
|
| Department |
Institute of Cancer
|
| Lab |
Centre for Medical Oncology
|
| Street address |
3rd Floor, John Vane Science Building, Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14926 |
Analysis of the impact of the method of cell selection on the gene expression profile of human CD4 and CD8 T cells |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE86362 |
