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Sample GSM3729010 Query DataSets for GSM3729010
Status Public on Apr 17, 2020
Title FB3: PBMC_BCG_wk3_FB
Sample type RNA
 
Source name Peripheral Blood, 3 weeks post-BCG
Organism Macaca mulatta
Characteristics gender: M
infant id: A16098-FB
week post-bcg timepoint: 3
bcg status: BCG-vaccinated
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Ficoll-paque gradient centrifugation was used to isolate 3.0EE6 macaque peripheral bllood mononuclear cells (PBMC) from EDTA-treated whole blood. Cellular RNA was extracted using the Macherey Nagel Nucleospin column purification system following the manufactureres instructions. PBMC were lysed by resuspending the 3.0EE6-cell pellat in 300µL of buffer RA1 with 1% beta-mercaptoethanol and storing long term at -80˚C. Lysed cells were thawed and RNA was purified prior to nanostring analysis.
Label NA
Label protocol NA
 
Hybridization protocol 100 nanograms of RNA was directly hybridized for 18 hours using the Non-human Primate Immunology v2.0 chipset.
Scan protocol Images were acquired from 280 fields for each sample
Data processing Raw data were processed in nSolver 4.0 using Advanced Analysis with R version 3.3.2 by running Normalization with the default settings of a threshold count value of 20 and an observation frequency of 1 with automatic selection of optimal house keeping genes.
 
Submission date Apr 17, 2019
Last update date Apr 18, 2020
Contact name Matthew Price Wood
E-mail(s) matthew.wood@seattlechildrens.org
Phone 2062568843239
Organization name Seattle Children's Research Institute
Department Global Infectious Diseases
Lab Sodora
Street address 307 Westlake Ave North
City Seattle
State/province WA
ZIP/Postal code 98106
Country USA
 
Platform ID GPL26516
Series (1)
GSE129989 BCG_CNRL_wk0vs3_infantMM

Data table header descriptions
ID_REF
VALUE Normalized fluorescent probe counts

Data table
ID_REF VALUE
PTGS1-mRNA 7.735238143
C1QBP-mRNA 9.251738764
MAFK-mRNA 3.358268685
NFE2L2-mRNA 8.270492725
SOCS1-mRNA 6.614608438
DOCK9-mRNA 7.67215946
TLR6-mRNA 7.528193686
STAT4-mRNA 7.3176267
CCL15-mRNA 5.275806524
C1QA-mRNA 3.888783401
C8B-mRNA 3.995698605
TSLP-mRNA 4.711905639
HMGB1-mRNA 12.16884032
MX1-mRNA 8.286375766
STAT2-mRNA 9.639554795
IL8-mRNA 12.87746809
ILF3-mRNA 10.62192912
DEFA1-mRNA 8.963130743
AREG-mRNA 6.647775302
IL22-mRNA 3.773306184

Total number of rows: 753

Table truncated, full table size 16 Kbytes.




Supplementary file Size Download File type/resource
GSM3729010_20181024_MW-2_FB3_10.RCC.gz 8.7 Kb (ftp)(http) RCC
Processed data included within Sample table

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