|
| Status |
Public on Apr 17, 2020 |
| Title |
FW3: PBMC_BCG_wk3_FW |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood, 3 weeks post-BCG
|
| Organism |
Macaca mulatta |
| Characteristics |
gender: F
infant id: A17154-FW
week post-bcg timepoint: 3
bcg status: unvaccinated
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ficoll-paque gradient centrifugation was used to isolate 3.0EE6 macaque peripheral bllood mononuclear cells (PBMC) from EDTA-treated whole blood. Cellular RNA was extracted using the Macherey Nagel Nucleospin column purification system following the manufactureres instructions. PBMC were lysed by resuspending the 3.0EE6-cell pellat in 300µL of buffer RA1 with 1% beta-mercaptoethanol and storing long term at -80˚C. Lysed cells were thawed and RNA was purified prior to nanostring analysis.
|
| Label |
NA
|
| Label protocol |
NA
|
| |
|
| Hybridization protocol |
100 nanograms of RNA was directly hybridized for 18 hours using the Non-human Primate Immunology v2.0 chipset.
|
| Scan protocol |
Images were acquired from 280 fields for each sample
|
| Data processing |
Raw data were processed in nSolver 4.0 using Advanced Analysis with R version 3.3.2 by running Normalization with the default settings of a threshold count value of 20 and an observation frequency of 1 with automatic selection of optimal house keeping genes.
|
| |
|
| Submission date |
Apr 17, 2019 |
| Last update date |
Apr 18, 2020 |
| Contact name |
Matthew Price Wood |
| E-mail(s) |
matthew.wood@seattlechildrens.org
|
| Phone |
2062568843239
|
| Organization name |
Seattle Children's Research Institute
|
| Department |
Global Infectious Diseases
|
| Lab |
Sodora
|
| Street address |
307 Westlake Ave North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98106 |
| Country |
USA |
| |
|
| Platform ID |
GPL26516 |
| Series (1) |
|
