|
| Status |
Public on Feb 14, 2010 |
| Title |
Adipose tissue 144 MUFA Baseline |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose tissue, monounsaturated fatty acid rich diet, baseline, individual 144
|
| Organism |
Homo sapiens |
| Characteristics |
individual: 144
tissue: Adipose
gender: Female
age: between 40-65 yrs
|
| Treatment protocol |
Subjects consumed a diet containing high SFA or high MUFA for 8 weeks. Subcutaneous adipose tissue samples were obtained by needle biopsy from the periumbilical area under local anesthesia both before and after the diet intervention, before and after the intervention.
|
| Growth protocol |
Twenty moderately overweight subjects, both males and females aged between 45-60 years, participated in this study. All subjects gave written informed consent to participate in the study and the study protocol was approved by the Medical Ethical Committee of Wageningen University, the Netherlands.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adipose tissue was rinsed with ice-cold phosphate-buffered saline to remove blood and immediately frozen in liquid nitrogen. Total RNA was isolated from adipose tissue using Trizol reagent (Invitrogen, Breda, NL) and purified using the Qiagen RNeasy Micro kit (Qiagen, Venlo, NL). RNA integrity was checked on an Agilent 2100 bioanalyzer (Agilent Technologies, Amsterdam, NL).
|
| Label |
biotin
|
| Label protocol |
The Ambion MessageAmp aRNA Amplification Kit was used to prepare labelled cRNA from 500ng of total RNA. The protocol was conducted using the reagents provided by Ambion (P/N 1751), as per the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridisation of 10ug cRNA was done overnight for 16 hours at 45ºC in a Hybridisation Oven 640 (Affymetrix). The protocol is conducted as described in the Genechip Expression Analysis Technical Manual, section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Target Hybridization) (P/N 701027, revision 5).
|
| Scan protocol |
Arrays were scanned on an Affymetrix 3000 7G scanner, as described n the Genechip Expression Analysis Technical Manual, section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Arrays: Washing, Staining and Scanning (P/N 701028, revision 5).
|
| Description |
n/a
|
| Data processing |
Expression estimates were calculated using RMA
|
| |
|
| Submission date |
Feb 23, 2009 |
| Last update date |
Apr 19, 2010 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL7020 |
| Series (1) |
| GSE14954 |
A saturated fatty acid rich diet can induce an 'obese-like' gene expression profile in adipose tissue |
|
