|
| Status |
Public on Apr 30, 2019 |
| Title |
RSM |
| Sample type |
SRA |
| |
|
| Source name |
Streptococcus pyogenes
|
| Organism |
Streptococcus pyogenes |
| Characteristics |
strain: MEW513
genotype: RM system deficient
|
| Growth protocol |
Cells were grown in C-media at 37C under anaerobic conditions to mid-log phase (OD600 of 0.6)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was purified using the Wizard Genomic DNA Purification Kit (Promega)
Library construction was performed by the University of Michigan Sequencing Core, 5kb mean insert size
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
PacBio RS II |
| |
|
| Data processing |
Library strategy: DNA-seq
Data processing was performed using RS_Modification_and_Motif_analysis.1 version 2.3.0 from SMRT portal
Genome_build: Streptococcus pyogenes MEW123, GenBank accession #: CP014139
Supplementary_files_format_and_content: HTML and text file with the identified modified motifs
|
| |
|
| Submission date |
Apr 29, 2019 |
| Last update date |
Apr 30, 2019 |
| Contact name |
Taylor Nye |
| E-mail(s) |
tnye@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
MCDB
|
| Lab |
Simmons Lab
|
| Street address |
1105 N University Ave
|
| City |
Ann Arbor |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL26590 |
| Series (2) |
| GSE130428 |
DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [PacBio] |
| GSE130429 |
DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes |
|
| Relations |
| BioSample |
SAMN11526126 |
| SRA |
SRX5763713 |
