|
Status |
Public on Apr 30, 2019 |
Title |
RSM |
Sample type |
SRA |
|
|
Source name |
Streptococcus pyogenes
|
Organism |
Streptococcus pyogenes |
Characteristics |
strain: MEW513 genotype: RM system deficient
|
Growth protocol |
Cells were grown in C-media at 37C under anaerobic conditions to mid-log phase (OD600 of 0.6)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was purified using the Wizard Genomic DNA Purification Kit (Promega) Library construction was performed by the University of Michigan Sequencing Core, 5kb mean insert size
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
PacBio RS II |
|
|
Data processing |
Library strategy: DNA-seq Data processing was performed using RS_Modification_and_Motif_analysis.1 version 2.3.0 from SMRT portal Genome_build: Streptococcus pyogenes MEW123, GenBank accession #: CP014139 Supplementary_files_format_and_content: HTML and text file with the identified modified motifs
|
|
|
Submission date |
Apr 29, 2019 |
Last update date |
Apr 30, 2019 |
Contact name |
Taylor Nye |
E-mail(s) |
tnye@umich.edu
|
Organization name |
University of Michigan
|
Department |
MCDB
|
Lab |
Simmons Lab
|
Street address |
1105 N University Ave
|
City |
Ann Arbor |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL26590 |
Series (2) |
GSE130428 |
DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes [PacBio] |
GSE130429 |
DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes |
|
Relations |
BioSample |
SAMN11526126 |
SRA |
SRX5763713 |