|
| Status |
Public on May 01, 2019 |
| Title |
Leaf, MIR160a CRISPR single nucleotide insertion line (T2), rep2 |
| Sample type |
SRA |
| |
|
| Source name |
leaf
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: leaf
ecotype: Col-0
genotype: MIR160a CRISPR single nucleotide insertion line (T2)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared using a Trizol reagent-based method
Small RNA libraries were made using 5 µg total RNA with TruSeq Small RNA Library Prep Kit, and sequenced on the Illumina HiSeq 2500 platform.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
mir160a*+1-2
|
| Data processing |
Sequenced reads were trimmed to remove adapters and converted to flat file format using the pre-processing script described in Mathioni et al. (DOI: 10.1002/cppb.20043).
Genome_build: None
Supplementary_files_format_and_content: Flat file with tab separated reads and abundance counts
|
| |
|
| Submission date |
Apr 30, 2019 |
| Last update date |
May 01, 2019 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL17639 |
| Series (1) |
| GSE130510 |
Disruption of miRNA Sequence by TALENs and CRISPR/Cas9 Induces Varied Length of miRNA Production [CRISPR/Cas9] |
|
| Relations |
| BioSample |
SAMN11539223 |
| SRA |
SRX5771362 |
