|
| Status |
Public on May 07, 2019 |
| Title |
ILC1a_3 |
| Sample type |
RNA |
| |
|
| Source name |
tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsil
cell type: ILC1a
markers: CD56+ NKp44+ CD196- CD300LF- CD103+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Briefly, tissue was mechanically disrupted, passed through 0.0059 inches opening sieve, and filtered extensively through 70μm cell strainers. CD56+ cells were enriched via positive selection with CD56 microbeads (Myltenyi), according to manufacturer’s recommendations. RNAs from the sorted ILC subsets were prepared by using RNeasy Plus Micro kit (Qiagen), following manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Purified RNA was amplified using Nugen Ovation Pico SL or Ovation Pico kits and labeled using Nugen Encore Biotin kit.
|
| |
|
| Hybridization protocol |
Products were hybridized to Affymetrix Human Gene 1.0 ST arrays according to the manufacturer’s protocol
|
| Scan protocol |
GS3000
|
| Data processing |
Data were analyzed with GenePattern software (Broad Institute). Raw data were normalized using standard RMA workflow.
|
| |
|
| Submission date |
May 06, 2019 |
| Last update date |
May 07, 2019 |
| Contact name |
Marco Colonna |
| E-mail(s) |
mcolonna@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE130773 |
Microarray data from human ILC transitional populations |
| GSE130775 |
Expression profiling of ILC transitional populations and Aiolos accessability and H3K27ac histone modifications in transfected MNK3 cells |
|
