|
| Status |
Public on May 09, 2019 |
| Title |
DMSO_vs_DAPT |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Endothelial Cells DMSO treated
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: 30hrs post fertilization
agent: DMSO
|
| Treatment protocol |
Tg(kdrl:GFP)s843; Tg(gata1:DsRed)sd2 embryos were incubated from 22 to 30 hpf with compounds that either promoted (DAPT 100µM), fully repressed (2.5 mM SU5415), or partially repressed (0.63 mM SU5416) angiogenic cell behavior in vivo.
|
| Growth protocol |
Tg(kdrl:GFP)s843; Tg(gata1:DsRed)sd2 embryos were obtained by natural mating and raised at 28ºC in E3 media until treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GFP-positive ECs were isolated by fluorescence-activated cell sorting (FACS) and separated from GFP/dsRed- double-positive erythrocytes directly into lusis buffer of RNAqueous-Micro kit (Thermofisher). Total RNA were extracted following manufacturer's guidelines.
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Channel 2 |
| Source name |
Endothelial cells DAPT treated
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: 30hrs post fertilization
agent: 100 uM DAPT
|
| Treatment protocol |
Tg(kdrl:GFP)s843; Tg(gata1:DsRed)sd2 embryos were incubated from 22 to 30 hpf with compounds that either promoted (DAPT 100µM), fully repressed (2.5 mM SU5415), or partially repressed (0.63 mM SU5416) angiogenic cell behavior in vivo.
|
| Growth protocol |
Tg(kdrl:GFP)s843; Tg(gata1:DsRed)sd2 embryos were obtained by natural mating and raised at 28ºC in E3 media until treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GFP-positive ECs were isolated by fluorescence-activated cell sorting (FACS) and separated from GFP/dsRed- double-positive erythrocytes directly into lusis buffer of RNAqueous-Micro kit (Thermofisher). Total RNA were extracted following manufacturer's guidelines.
|
| Label |
Cy5
|
| Label protocol |
standard Agilent protocol
|
| |
|
| |
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
Slides were scanned using Agilent Technologies Scanner G2505C and protocol GE2_107
|
| Data processing |
Normalization and data control using GeneSpring GX software
|
| |
|
| Submission date |
May 08, 2019 |
| Last update date |
May 09, 2019 |
| Contact name |
Shane P Herbert |
| E-mail(s) |
shane.herbert@manchester.ac.uk
|
| Organization name |
University of Manchester
|
| Department |
Faculty of Biology, Medicine and Health
|
| Lab |
Division of Developmental Biology and Medicine
|
| Street address |
Michael Smith Building, OXford Road
|
| City |
Manchester |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE130889 |
Pro-angiogenic transcriptome of zebrafish endothelial cells |
|
