Data table header descriptions |
ID_REF |
|
X |
X coordinate |
Y |
Y coordinate |
Dia. |
Spot diameter |
CH1_RAW |
Cy5 intensity (median) |
F635 Mean |
Cy5 intensity (mean) |
CH1_SD |
Cy5 intensity stdev |
CH1_BKD_RAW |
Cy5 background (median) |
B635 Mean |
Cy5 background (mean) |
CH1_BKD_SD |
Cy5 background stdev |
% > B635+1SD |
Cy5 background error 1 |
% > B635+2SD |
CY5 background error 2 |
F635 % Sat. |
CY5 percentage of saturated pixels |
CH2_RAW |
Cy3 intensity (median) |
F532 Mean |
Cy3 intensity (mean) |
CH2_SD |
Cy5 intensity stdev |
CH2_BKD_RAW |
3 background (median) |
B532 Mean |
Cy3 background (mean) |
CH2_BKD_SD |
Cy3 background stdev |
% > B532+1SD |
Cy3 background error 1 |
% > B532+2SD |
Cy3 background error 2 |
F532 % Sat. |
Cy3 percentage of saturated pixels |
Ratio of Medians (635/532) |
Ratio of medians |
Ratio of Means (635/532) |
Ratio of means |
Median of Ratios (635/532) |
Median of ratios |
Mean of Ratios (635/532) |
Mean of ratios |
Ratios SD (635/532) |
Ratio stdev |
Rgn Ratio (635/532) |
Regression ratio |
Rgn R? (635/532) |
Regression ratio squared |
F Pixels |
Foreground pixels |
B Pixels |
Background pixels |
Sum of Medians |
Sum of medians |
Sum of Means |
Sum of means |
Log Ratio (635/532) |
Log ratio Cy5/Cy3 (genepix) |
F635 Median - B635 |
Cy5 background corrected (median) |
F532 Median - B532 |
Cy3 background corrected (median) |
F635 Mean - B635 |
Cy5 background corrected (mean) |
F532 Mean - B532 |
Cy3 background corrected (mean) |
Flags |
Spot quality |
VALUE |
Log2 normalised ratio Cy5/Cy3 |