|
| Status |
Public on Oct 28, 2020 |
| Title |
RNA-seq_OS5K_1 |
| Sample type |
SRA |
| |
|
| Source name |
OS5K #1
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: untreated
histology: clear cell renal cell carcinoma
|
| Treatment protocol |
untreated
|
| Growth protocol |
OS-RC-2 was obtained from RIKEN Cell Bank (Tsukuba, Japan) and cultured at 37℃ and 5% CO2 in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA) containing 10% (v/v) foetal bovine serum (FBS) (GE Healthcare Life Sciences, Piscataway, NJ).
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated from cultured cells with RNeasy (Qiagen, Venlo, Netherlands).
cDNA libraries were prepared using Dynabeads mRNA DIRECT Purification Kit (Thermo Fisher Scientific) and Ion Total RNA-Seq Kit v.2 (Thermo Fisher Scientific).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Description |
RNA-seq of cultured OS5K #1
|
| Data processing |
Reads per kilobase of exon permMegabase of library size (RPKM) were calculated by CLC Genomics Workbench (Qiagen).
Genome_build: hg19
Supplementary_files_format_and_content: Expression data of the four samples were summarized into a processed file.
|
| |
|
| Submission date |
May 13, 2019 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Shogo Ehata |
| E-mail(s) |
ehata-jun@umin.ac.jp
|
| Organization name |
The University of Tokyo
|
| Department |
Graduate School of Medicine
|
| Lab |
Deaprtment of Molecular Pathology
|
| Street address |
Hongo 7-3-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE131137 |
RNA-sequencing of a clear cell renal cell carcinoma cell line, OS-RC-2 |
|
| Relations |
| BioSample |
SAMN11637229 |
| SRA |
SRX5827053 |
