|
Status |
Public on Oct 28, 2020 |
Title |
RNA-seq_OS5K_2 |
Sample type |
SRA |
|
|
Source name |
OS5K #2
|
Organism |
Homo sapiens |
Characteristics |
treatment: untreated histology: clear cell renal cell carcinoma
|
Treatment protocol |
untreated
|
Growth protocol |
OS-RC-2 was obtained from RIKEN Cell Bank (Tsukuba, Japan) and cultured at 37℃ and 5% CO2 in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA) containing 10% (v/v) foetal bovine serum (FBS) (GE Healthcare Life Sciences, Piscataway, NJ).
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was isolated from cultured cells with RNeasy (Qiagen, Venlo, Netherlands). cDNA libraries were prepared using Dynabeads mRNA DIRECT Purification Kit (Thermo Fisher Scientific) and Ion Total RNA-Seq Kit v.2 (Thermo Fisher Scientific).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Ion Torrent Proton |
|
|
Description |
RNA-seq of cultured OS5K #2
|
Data processing |
Reads per kilobase of exon permMegabase of library size (RPKM) were calculated by CLC Genomics Workbench (Qiagen). Genome_build: hg19 Supplementary_files_format_and_content: Expression data of the four samples were summarized into a processed file.
|
|
|
Submission date |
May 13, 2019 |
Last update date |
Oct 28, 2020 |
Contact name |
Shogo Ehata |
E-mail(s) |
ehata-jun@umin.ac.jp
|
Organization name |
The University of Tokyo
|
Department |
Graduate School of Medicine
|
Lab |
Deaprtment of Molecular Pathology
|
Street address |
Hongo 7-3-1, Bunkyo-ku
|
City |
Tokyo |
ZIP/Postal code |
113-0033 |
Country |
Japan |
|
|
Platform ID |
GPL17303 |
Series (1) |
GSE131137 |
RNA-sequencing of a clear cell renal cell carcinoma cell line, OS-RC-2 |
|
Relations |
BioSample |
SAMN11637228 |
SRA |
SRX5827054 |