|
| Status |
Public on Sep 25, 2019 |
| Title |
Lung428 |
| Sample type |
SRA |
| |
|
| Source name |
PO Betamethasone-phosphate_Lung
|
| Organism |
Macaca mulatta |
| Characteristics |
gestational age: 131 days
treatment route and drug: PO Betamethasone-phosphate
dose: 0.15mg/kg x3 12h apart
treatment to delivery interval: 5 days
tissue: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol per manufacturer's guidelines.
Afer polyA selection, paired-end RNA-sequencing with 75bp was done with a depth of 30M reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
processed data file:
countslung.csv
run0064_lane91_read*_indexD712-D506=RhLung428-18
|
| Data processing |
Alignment to the MMUL8.01 done with STAR
Read counts was done using FeatureCounts
Differential expression analysis was performed with DESEq2
Genome_build: MMUL8.01
Supplementary_files_format_and_content: counts
|
| |
|
| Submission date |
May 14, 2019 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Augusto F Schmidt |
| E-mail(s) |
aschmidt@med.miami.edu
|
| Phone |
3052430156
|
| Organization name |
University of Miami Miller School of Medicine
|
| Street address |
1611 NW 12th ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL14954 |
| Series (1) |
| GSE131194 |
Oral dosing for antenatal corticosteroids in the rhesus macaque |
|
| Relations |
| BioSample |
SAMN11640951 |
| SRA |
SRX5830476 |
