|
| Status |
Public on Jul 13, 2020 |
| Title |
JS2_R2 |
| Sample type |
SRA |
| |
|
| Source name |
Seed
|
| Organism |
Cicer arietinum |
| Characteristics |
genotype: JGK 3
tissue: Seed
development stage: Mid embryogenesis (S2)
|
| Growth protocol |
Plants were grown in natural field conditions and seeds undergoing different stages of development were harvested from field-grown plants.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated followed by preparation of small RNA sequencing libraries using TruSeq Small RNA Sample Prep Kit (Illumina Technologies).
Small RNA libraries were prepared for sequencing using Illumina's standard protocols.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
S2_R2
|
| Data processing |
Data obtained in FASTQ files were processed to remove adapter sequences using cutadapt (v1.12) and low-quality reads using qrqc package in R.
High-quality filtered identical reads were collapsed into a unique reads to estimate abundance of small RNAs at different stages of seed development and leaf tissue.
Supplementary_files_format_and_content: FASTA files containing non-redundant reads with their read counts.
|
| |
|
| Submission date |
May 17, 2019 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Mukesh Jain |
| Phone |
91-11-26704686
|
| Organization name |
Jawaharlal Nehru University
|
| Street address |
New Mehrauli Road
|
| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL20619 |
| Series (1) |
| GSE131424 |
Small RNAs during seed development in large-seeded chickpea |
|
| Relations |
| BioSample |
SAMN11672519 |
| SRA |
SRX5860043 |
