|
| Status |
Public on Mar 06, 2009 |
| Title |
NCI-H460 |
| Sample type |
RNA |
| |
|
| Source name |
Lung:NSCLC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H460
tissue: Lung:NSCLC
k-ras dependence: independent
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylation of RNA with Perkin Elmer PN Biotin-11-UTP, and Biotin-11-CTP)
|
| |
|
| Hybridization protocol |
Labelled RNA was processed using a protocol from Affymetrix's GeneChip Expression Analysis Technical Manual under Eukaryotic Sample and Array Processing
|
| Scan protocol |
Scanning was performed with an Affymetrix GeneChip 3000 Scanner
|
| Description |
Gene expression data
|
| Data processing |
Data was processed with GCOS ver 1.4
|
| |
|
| Submission date |
Mar 05, 2009 |
| Last update date |
Mar 05, 2009 |
| Contact name |
Patricia Greninger |
| E-mail(s) |
pgreninger@partners.org
|
| Organization name |
Massachusetts General Hospital Cancer Center
|
| Department |
Center for Molecular Therapeutics
|
| Lab |
Settleman Lab
|
| Street address |
149 13th St
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE15126 |
A gene expression signature associated with K-Ras addiction reveals regulators of EMT and tumor cell survival |
|
