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Sample GSM378141 Query DataSets for GSM378141
Status Public on Mar 06, 2009
Title T47D cell line
Sample type genomic
 
Channel 1
Source name germline DNA from anonymous donor
Organism Homo sapiens
Characteristics tissue: blood
gender: female
Treatment protocol Normal growing conditions.
Growth protocol Grown under subconfluent conditions in growth media (RPMI 1640, pyruvate, 10 mM Hepes, glucose to 4.5g/L, 10% FBS, antibiotics).
Extracted molecule genomic DNA
Extraction protocol Qiagen QiaAmp DNA Micro kit.
Label Cy3
Label protocol Agilent Genomic DNA Labeling kit.
 
Channel 2
Source name T47D cell line
Organism Homo sapiens
Characteristics cell line: T47D
Treatment protocol Normal growing conditions.
Growth protocol Grown under subconfluent conditions in growth media (RPMI 1640, pyruvate, 10 mM Hepes, glucose to 4.5g/L, 10% FBS, antibiotics).
Extracted molecule genomic DNA
Extraction protocol Qiagen QiaAmp DNA Micro kit.
Label Cy5
Label protocol Agilent Genomic DNA Labeling kit.
 
 
Hybridization protocol Agilent Direct Method.
Scan protocol Agilent Scan Control Software.
Description T47D
Data processing A process control algorithm was used to flag log cgh probe ratios and calculate Z' scores. Z' scores >=2 were used to identify probable copy gains and Z' scores <= -2 were used to identify probable copy loss. Z' scores for the genes of interest are available on the Series record.
 
Submission date Mar 05, 2009
Last update date Dec 26, 2023
Contact name Cynthia Ma
E-mail(s) cynthiaxma@wustl.edu
Phone 3143628903
Organization name Washington University
Department Medical Oncology
Lab Dr. Cynthia Ma Lab
Street address 4515 Mckinley, 4515 Mckinley
City st louis
State/province Missouri
ZIP/Postal code 63627
Country USA
 
Platform ID GPL4091
Series (2)
GSE15131 PIK3CB aCGH Cell Line Results
GSE15134 PI3K inhibition in ER+ breast cancer microarray data

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -5.526804664e-002
2 -2.888845935e-001
3 0.000000000e+000
4 4.591393814e-002
5 5.838609625e-002
6 -1.957858549e-001
7 2.618487832e-001
8 -3.464667345e-002
9 1.677679799e-001
10 -1.302881629e-001
11 4.858483170e-001
12 1.897073013e-001
13 1.457849476e-002
14 -1.794702442e-002
15 4.066310445e-001
16 -2.551464337e-001
17 1.686050694e-001
18 1.263033234e-001
19 -2.192535789e-004
20 1.289478239e-001

Total number of rows: 243430

Table truncated, full table size 5714 Kbytes.




Supplementary file Size Download File type/resource
GSM378141.txt.gz 67.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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