|
Status |
Public on Mar 06, 2009 |
Title |
HCC1937 cell line |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
germline DNA from anonymous donor
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood gender: female
|
Treatment protocol |
Normal growing conditions.
|
Growth protocol |
Grown under subconfluent conditions in growth media (RPMI 1640, pyruvate, 10 mM Hepes, glucose to 4.5g/L, 10% FBS, antibiotics).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Qiagen QiaAmp DNA Micro kit.
|
Label |
Cy3
|
Label protocol |
Agilent Genomic DNA Labeling kit.
|
|
|
Channel 2 |
Source name |
HCC1937 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCC1937
|
Treatment protocol |
Normal growing conditions.
|
Growth protocol |
Grown under subconfluent conditions in growth media (RPMI 1640, pyruvate, 10 mM Hepes, glucose to 4.5g/L, 10% FBS, antibiotics).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Qiagen QiaAmp DNA Micro kit.
|
Label |
Cy5
|
Label protocol |
Agilent Genomic DNA Labeling kit.
|
|
|
|
Hybridization protocol |
Agilent Direct Method.
|
Scan protocol |
Agilent Scan Control Software.
|
Description |
HCC1937
|
Data processing |
A process control algorithm was used to flag log cgh probe ratios and calculate Z' scores. Z' scores >=2 were used to identify probable copy gains and Z' scores <= -2 were used to identify probable copy loss. Z' scores for the genes of interest are available on the Series record.
|
|
|
Submission date |
Mar 05, 2009 |
Last update date |
Dec 26, 2023 |
Contact name |
Cynthia Ma |
E-mail(s) |
cynthiaxma@wustl.edu
|
Phone |
3143628903
|
Organization name |
Washington University
|
Department |
Medical Oncology
|
Lab |
Dr. Cynthia Ma Lab
|
Street address |
4515 Mckinley, 4515 Mckinley
|
City |
st louis |
State/province |
Missouri |
ZIP/Postal code |
63627 |
Country |
USA |
|
|
Platform ID |
GPL4091 |
Series (2) |
GSE15131 |
PIK3CB aCGH Cell Line Results |
GSE15134 |
PI3K inhibition in ER+ breast cancer microarray data |
|