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Sample GSM379835 Query DataSets for GSM379835
Status Public on Jun 30, 2011
Title Patient2_339_GH_Day2_GH1_11AM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day2_GH1_11AM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) mmaningat@mail.rockefeller.edu
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 164.539 34 8.548409 0.001412429
ILMN_1660305 352.3246 47 9.768144 0
ILMN_1792173 175.5754 49 6.042621 0.001412429
ILMN_1762337 59.55545 35 2.22173 0.7344633
ILMN_1736007 66.06944 43 2.364119 0.3870057
ILMN_1787689 74.19524 35 3.904064 0.1144068
ILMN_1731507 54.05453 35 2.229356 0.9519774
ILMN_1814316 68.25616 35 3.363644 0.2951977
ILMN_1745607 66.36166 47 2.569301 0.3686441
ILMN_1757454 60.2318 40 2.654592 0.7019774
ILMN_1735045 76.85991 42 4.689192 0.08615819
ILMN_1680754 82.05663 36 4.284221 0.04943503
ILMN_1659452 62.24436 39 2.188115 0.5677966
ILMN_1767388 140.4905 48 4.300922 0.002824859
ILMN_1673870 69.93775 44 3.038025 0.2372881
ILMN_1675204 58.31277 36 2.616912 0.7980226
ILMN_1755321 144.1419 43 4.798273 0.001412429
ILMN_1698554 1570.446 43 36.941 0
ILMN_1805938 63.26749 50 2.609612 0.5183616
ILMN_1676336 85.63937 41 3.585845 0.03248588

Total number of rows: 22184

Table truncated, full table size 912 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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