|
| Status |
Public on Oct 02, 2009 |
| Title |
CD4 T-cell responder rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Rheumatoid arthritis
cell type: CD4 T cells
phenotype: Rituximab responder
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction using Rneasy Kit (Qiagen) from CD4 T cell isolates preserved in RNALater.
|
| Label |
Biotin
|
| Label protocol |
Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
|
| |
|
| Hybridization protocol |
Biotin labeled cRNA was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
|
| Scan protocol |
Scanning was performed on the Illumina(r) BeadStation 500x.
|
| Description |
Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Isolated whole blood CD4 T cells, rituximab responder
|
| Data processing |
Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
|
| |
|
| Submission date |
Mar 19, 2009 |
| Last update date |
Oct 02, 2009 |
| Contact name |
Antonio Julià |
| E-mail(s) |
toni.julia@vhir.org
|
| Phone |
+34 934029082
|
| Organization name |
Vall d'Hebron Hospital Research Institute
|
| Department |
Rheumatology Research Group
|
| Lab |
Rheumatology Research Group
|
| Street address |
c/ Baldiri Reixac nº15
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE15316 |
Differential expression of rituximab responders vs. non responders on 3 different blood cell types |
|
