|
| Status |
Public on Nov 14, 2019 |
| Title |
KOV4: Long-term survivor (HG-U133A) |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ovary
tumor stage: IIB
tumor histotype: high grade serous
phenotype: platinum sensitive
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified from snap-frozen biopsies containing at least 70% of tumor epithelial cells by TRIzolⓇ Reagent (ThermoFisher) according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 °C on GeneChip® Human Genome U133A oligonucleotide microarray chips (Affymetrix, Inc., Santa Clara, CA, USA) following the manufacturer’s protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Affymetrix Gene Chip Command Console Software, using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 30, 2019 |
| Last update date |
Nov 15, 2019 |
| Contact name |
Antonella Ravaggi |
| E-mail(s) |
antonella.ravaggi@unibs.it
|
| Organization name |
University of Brescia
|
| Department |
Department of Clinical and Experimental Sciences
|
| Lab |
Division of Obstetrics and Gynecology
|
| Street address |
Viale Europa 11
|
| City |
Brescia |
| State/province |
Brescia |
| ZIP/Postal code |
25123 |
| Country |
Italy |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE131978 |
FXYD5 (dysadherin) upregulation predicts shorter survival and reveals platinum resistance in high-grade serous ovarian cancer |
|
