organ/tissue: prostate tissue preparation: cell line cell type: carcinoma other information: LNCaP cells were maintained in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Inc., Gaithersburg, MD) and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS (cFBS, Gemini Bio-Products, Inc., Calabasas, CA) was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD).
Biomaterial provider
LNCaP cells (Item Number CRL-1740, American Type Culture Collection, Rockville, MD)
Extracted molecule
total RNA
Extraction protocol
N/A
Description
CGAP_Numeric_library_ID: 69 Tagging_enzyme: BsmF I Old_library_name: SAGE_CPDR_LNCaP-C Library_producer: L. L. Xu Laboratory: S. Srivastava, Uniformed Services University of the Health Sciences References: L. L. Xu et al., Genomics 66, 257-63 (2000) [PUBMED=10873380]|L. L. Xu et al., Int J Cancer 92, 322-8 (2001) [PUBMED=11291065]
